Malaria Pf Pv Rapid Kwayedza Kit

Pfupiso uye KUTSANANGURWA KWEMAEDZO

Malaria chirwere chinotakurwa neumhutu, hemolytic, febrile icho chinobata vanhu vanopfuura mamirioni mazana maviri uye chinouraya vanopfuura miriyoni imwe pagore. Inokonzereswa nemhando ina dzePlasmodium: P. falciparum, P. vivax, P. ovale, uye P. malariae.

Iyo Malaria Pf / Pv Ag Rapid Test inoshandisa antibodies yakanangana naP. Falciparum Histidine Rich Protein-II (pHRP-II) uye kuna P. vivax Lactate Dehydrogenase (Pv-LDH) kuona pamwe nekusiyanisa hutachiona naP. Falciparum naP. vivax-5. Muedzo unogona kuitwa nevasina kudzidziswa kana vashoma vane hunyanzvi vashandi, vasina michina yemarabhoritari

MUedzo WEMAZANO

Iyo Malaria Pf / Pv Ag Inokurumidza Bvunzo ndeye lateral kuyerera chromatographic immunoassay. Iyo bhendi bvunzo yezvikamu ine: 1) burgundy yakavezwa conjugate pad ine mbeva anti-Pv-LDH antibody yakasanganiswa necolloid goridhe (Pv-LDH-goridhe conjugates) uye mbeva anti-pHRP-II antibody yakasanganiswa necolloid goridhe (pHRP-II -gold conjugates), 2) nitrocellulose membrane tambo ine maviri bvunzo mabhendi (Pv uye Pf mabhendi) uye inodzora bhendi (C bhendi). Iyo Pv bhendi pre-yakavharwa neimwe mbeva inorwisa-Pv-LDH yakatarisana antibody yekuonekwa kwePv hutachiona, iyo Pf bhendi yakagara yakaputirwa ne polyclonal anti-pHRP-II antibodies yekuonekwa kwePf hutachiona, uye iyo C band akafukidzwa nembudzi inopesana negonzo IgG.

REAGENTS NEZVINHU ZVINOPIWA

1.Chikwata chega chega chine zvishandiso zvekuyedza makumi maviri neshanu, imwe neimwe yakavharirwa muhomwe yezvinyorwa ine zvinhu zvitatu mukati

a. Imwe kaseti kadhi.
b. Imwe desiccant.

2. 25 x 5 µL mini mapurasitiki anodonhedza

3. Ropa Lysis buffer (1 bhodhoro, 10 mL)

4.One package package (kuraira kwekushandisa).

KUCHENGETA NOKUDA-HERE

1. Chengetera bvunzo kifaa chakaputirwa mune yakasimbiswa foil homwe pa2-30 ℃ (36-86F) .Usagadzire.

2. Sirifu-hupenyu: mwedzi makumi maviri nemana kubva pazuva rekugadzira.

ASSAY MAITIRO

Nhanho 1: Uya nemuenzaniso uye zviyero zvekuyedza kutonhorera remukati kana firiji kana chando.

Sanganisa iwo mushe mushe musati wamboedzwa kamwe kanyungudutswa. Ropa richaputirwa mushure mekunyungudika.

Nhanho 2: Kana wagadzirira kuyedza, vhura homwe pane notch uye bvisa chishandiso. Isa chimbo chekuyedza

panzvimbo yakachena, yakati sandara.

Nhanho 3: Ive neshuwa yekunyorera chishandiso ine specimen's ID nhamba.

Nhanho 4: Zadza mini mini epurasitiki dhizaini neropa specimen kuti usapfuure iyo specimen mutsara sezvakaratidzwa mumufananidzo unotevera Huwandu hweiyo specimen hwakatenderedza 5 aroundL.

Kubata iyo inodonhedza yakatwasuka, govera zvese zvifananidziro pakati peiyo sampuro mushe uchiita chokwadi chekuti hapana mabhuru emhepo.

Wobva wawedzera matatu madonhwe (anenge 100-150 µL) eLysis Buffer nekukasira.

Nhanho 5: Seta timer.

Nhanho 6: Mhedzisiro inogona kuverengerwa mumaminetsi makumi maviri kusvika makumi matatu. Zvinotora maminetsi anodarika makumi maviri kuti mamiriro acho anyatsojeka.

Usaverenge zvawanikwa mushure memaminitsi makumi matatu. Kuti udzivise kuvhiringidzika, rasa bvunzo mudziyo mushure mekududzira mhedzisiro.

DUDZIRO YEMHARA

1. NEGATIVE RESULT: Kana chete C bhendi iripo, kusavapo kweye chero burgundy ruvara mune ese bvunzo bhendi (Pv nePf) kunoratidza kuti hapana anti-plasmodium antigen inoonekwa. Mhedzisiro yacho haina kunaka.

2. ZVINOGONESESA

2.1 Mukuwedzera pakuvapo kweC band, kana chete Pv bhendi yakagadziriswa, iyo bvunzo inoratidza kuvepo kwePv-LDH antigen. Mhedzisiro yacho Pv yakanaka.

2.2 Pamusoro pehupo hweC band, kana chete Pf bhendi yakagadziridzwa, bvunzo inoratidza kuvepo kwepHRP-II antigen. Mhedzisiro yacho Pf yakanaka.

2.3 Kunze kwekuvapo kweC band, ese maPv nemaPv mabhendi anovandudzwa, iyo bvunzo inoratidza kuvepo kweese maviri Pv-LDH uye pHRP-II maantigen. Mhedzisiro yacho zvese zviri zviviri Pv uye Pf yakanaka.

3. INVALID: Kana pasina C bhendi rakagadzirwa, iyo assay haina kukodzera zvisinei chero burgundy ruvara mune bvunzo mabhendi sezvakaratidzwa pazasi. Dzokorora chiyero nechigadzirwa chitsva.